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Hi to all,
I read the file myDB.mdb with :

strConnString ="Provider=Microsoft.Jet.OLEDB.4.0; Data Source=C:\myfile.mdb";
OleDbConnection conn = new OleDbConnection(strConnString);
conn.Open();
..........
all ok.

Then I have changed the Web.config file so :

<authentication mode="Windows"/>
<identity impersonate="true"/>

<allow users="machineName\myUser"/>
<deny users="*" />

and I grant only "myUser" on the database folder with all permissions.

Now on the conn.Open() I have the following OledbException :

messagge = error not specify
source = "Microsoft JET Database Engine"

Where is the error ?

Thanks to all