1. Check if you have created the virtual directory for your application or delete the existing virtual directory, create a new Virtual directory, restart IIS and access your page.
If the problem persist, check the permission on your the following folder:
%WINDIR%\Microsoft.NET\Framework\v1.1.4322\Tempora ry ASP.NET Files
This folder should have 'full control' for LOCAL SERVICE,NETWORK SERVICE and the root folder of you website (e.g.WWWROOT) should have the 'full control' permission for LOCAL SERVICE, NETWORK SERVICE.
One of these options might help you to resolve.
Thanks,
R
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